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Check the network settings: Are you able to ping the AnywhereUSB Plus hub's IP address? Can you open the Hub's Dashboard page with a web browser, or SSH to the CLI of the Hub at that IP adderess?
Additional troubleshooting steps: Verifying the connection with the Hub: https://www.digi.com/resources/documentation/digidocs/90002383/default.htm#device/anywhereusbplus/content/tasks/t_initial_connection.htm Be sure to hit "Apply" after changing the Admin password in the following step: https://www.digi.com/resources/documentation/digidocs/90002383/default.htm#device/anywhereusbplus/content/tasks/t_change_default_pw_hub.htm Common causes of an "Unable to connect" status:
https://www.digi.com/resources/documentation/digidocs/90002383/default.htm#device/anywhereusbplus/content/troubleshooting/ts_unable_to_connect.htm
If the above recommendation doesn't resolve the USB application error, please contact Digi Technical Support for further assistance.